LOCUS       sequence-147089-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mKlf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   dos Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, 
            Hendrich B, Silva JC
  TITLE     MBD3/NuRD facilitates induction of pluripotency in a 
            context-dependent manner.
  JOURNAL   Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 
            10.1016/j.stem.2014.04.019. Epub 2014 May 15.
  PUBMED    24835571
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2014.04.019"; journalName: "Cell Stem Cell"; date: 
            "2014-07-3- 3"; volume: "15"; issue: "1"; pages: "102-10"
COMMENT     Sequence Label: pPB-CAG-Klf4-pA-pgk-hph
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..47
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(62..67,68..85,86..92)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     124..140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
       61 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      121 acacaggaaa cagctatgac catgattacg ccaagctatc accactttgt acaagaaagc
      181 tgggtcggcg cgcccaccct tacatccact acgtgggatt taaaagtgcc tcttcatgtg
      241 taaggcaagg tggtccgacc tggaaaaggc cctgtcgcac ttctggcact gaaagggccg
      301 gtgccctgtg tgtttgcggt agtgcctggt cagttcatcg gagcgggcga atttccaccc
      361 acagccgtcc cagtcacagt ggtaaggttt ctcgcctgtg tgagttcgca ggtgtgcctt
      421 gagatgagaa ctcttggtat aggttttgcc acagcctgca tagtcacaag tgtgggtggc
      481 tgttcttttc cggggccacg accttcttcc cctctttggc ttgggctcct ctggcaggca
      541 ggaacccggt ggcatgagct cttgataatg gagagagggg acttgtgact gcatctggtc
      601 tggcaggaaa ggagggtagt tgggccccgg atggggatgg aatcctgggg gaagaggcag
      661 gccagggtga cagtccctgc tgttcagcag ttcctcggga ctcagtgtag gggtagtcct
      721 ggtggggagc tgccgcccca gggggaagtc gtgtgtgttg ggccggtggc cgttgctgag
      781 ctggggtcca gcgctcaaat gggcctctag ggaccggctg accgtgcagg acgggaccgc
      841 ctcttgctta atcttggggc acatgcgcgg cgggccaccg ctgtagggcg ccactaccac
      901 ggggtggctg ccgtctgggc ttcctttgct aacactgatg ac
//
