LOCUS       sequence-147095-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid against human Ikaros.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(20..268)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(78..97)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(248..268)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(410..429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaagcgata ctccagaccg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat
       61 cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa
      121 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
      181 tgtttacagt caaattaatt ccaattatct ctctaacagc cttgtatcgt atatgcaaat
      241 atgaaggaat catgggaaat aggccctcac atgtgagcaa aaggccagca aaaggccagg
      301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag g
//
