LOCUS       sequence-147098-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-cNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..47
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(62..67,68..85,86..92)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     124..140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
       61 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      121 acacaggaaa cagctatgac catgattacg ccaagctatc accactttgt acaagaaagc
      181 tgggtcctaa gtctcataac cattctgggt ccccagggta tgccaagggg cctggtactg
      241 atgccgtaca ggagagctcg agaactgtgt gatactatca gaggtgctct ggaagctgta
      301 ggtatcttct gcctgcaagc tgtcataatc cacattggta gagtagccat gatagaagtt
      361 catctgttga cttaataaac ccatggcctg ctgggcgttg cagcttgttc caccttttcc
      421 aaagaagccc tcatcctcca cagccatgaa cggatacagg ccctgcccat tcccataagt
      481 ctggccactg ctgtaagcct ggtgtgcact ggtcacagcc tgaaggtttc tgttggcaac
      541 aacagggaag ccctggtgaa acgtaggggt catatccaga tacgcagctt gatgaaaccc
      601 attctgtggt agataaatcc ctttatccac ccactgactc tctttctggc aacgtttaaa
      661 cttcattcgt tggttctgaa accacgtctt cacctgcttg taggtgagcc ccagagcagc
      721 agccagctcc cggatctgat gggggctgag gtacttctgg ctctgaaacc gctggtgcag
      781 ggtctgcagc tgctcctggg agaaagctgt gcggctcttg gccttccttg tcccactctc
      841 acctt
//
