LOCUS       sequence-147105-        3539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..3539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2780..2789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2786..3499
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgcacca tcatggacca
       61 ttatgattct cagcaaacca acgattacat gcagccagaa gaggactggg accgggacct
      121 gctcctggac ccggcctggg agaagcagca gagaaagaca ttcacggcat ggtgtaactc
      181 ccacctccgg aaggcgggga cacagatcga gaacatcgaa gaggacttcc gggatggcct
      241 gaagctcatg ctgctgctgg aggtcatctc aggtgaacgc ttggccaagc cagagcgagg
      301 caagatgaga gtgcacaaga tctccaacgt caacaaggcc ctggatttca tagccagcaa
      361 aggcgtcaaa ctggtgtcca tcggagccga agaaatcgtg gatgggaatg tgaagatgac
      421 cctgggcatg atctggacca tcatcctgcg ctttgccatc caggacatct ccgtggaaga
      481 gacttcagcc aaggaagggc tgctcctgtg gtgtcagaga aagacagccc cttacaaaaa
      541 tgtcaacatc cagaacttcc acataagctg gaaggatggc ctcggcttct gtgctttgat
      601 ccaccgacac cggcccgagc tgattgacta cgggaagctg cggaaggatg atccactcac
      661 aaatctgaat acggcttttg acgtggcaga gaagtacctg gacatcccca agatgctgga
      721 tgccgaagac atcgttggaa ctgcccgacc ggatgagaaa gccatcatga cttacgtgtc
      781 tagcttctac cacgccttct ctggagccca gaaggcggag acagcagcca atcgcatctg
      841 caaggtgttg gccgtcaacc aggagaacga gcagcttatg gaagactacg agaagctggc
      901 cagtgatctg ttggagtgga tccgccgcac aatcccgtgg ctggagaacc gggtgcccga
      961 gaacaccatg catgccatgc aacagaagct ggaggacttc cgggactacc ggcgcctgca
     1021 caagccgccc aaggtgcagg agaagtgcca gctggagatc aacttcaaca cgctgcagac
     1081 caagctgcgg ctcagcaacc ggcctgcctt catgccctct gagggcagga tggtctcgga
     1141 catcaacaat gcctggggct gcctggagca ggtggagaag ggctatgagg agtggttgct
     1201 gaatgagatc cggaggctgg agcgactgga ccacctggca gagaagttcc ggcagaaggc
     1261 ctccatccac gaggcctgga ctgacggcaa agaggccatg ctgcgacaga aggactatga
     1321 gaccgccacc ctctcggaga tcaaggcctt gctcaagaag catgaggcct tcgagagtga
     1381 cctggctgcc caccaggacc gtgtggagca gattgccgcc atcgcacagg agctcaatga
     1441 gctggactat tatgactcac ccagtgtcaa cgcccgttgc caaaagatct gtgaccagtg
     1501 ggacaatctg ggggccctaa ctcagaagcg aagggaagct ctggagcgga ccgagaaact
     1561 gctggagacc attgaccagc tgtacttgga gtatgccaag cgggctgcac ccttcaacaa
     1621 ctggatggag ggggccatgg aggacctgca ggacaccttc attgtgcaca ccattgagga
     1681 gatccaggga ctgaccacag cccatgagca gttcaaggcc accctccctg atgccgacaa
     1741 ggagcgcctg gccatcctgg gcatccacaa tgaggtgtcc aagattgtcc agacctacca
     1801 cgtcaatatg gcgggcacca acccctacac aaccatcacg cctcaggaga tcaatggcaa
     1861 atgggaccac gtgcggcagc tggtgcctcg gagggaccaa gctctgacgg aggagcatgc
     1921 ccgacagcag cacaatgaga ggctacgcaa gcagtttgga gcccaggcca atgtcatcgg
     1981 gccctggatc cagaccaaga tggaggagat cgggaggatc tccattgaga tgcatgggac
     2041 cctggaggac cagctcagcc acctgcggca gtatgagaag agcatcgtca actacaagcc
     2101 aaagattgat cagctggagg gcgaccacca gctcatccag gaggcgctca tcttcgacaa
     2161 caagcacacc aactacacca tggagcacat ccgtgtgggc tgggagcagc tgctcaccac
     2221 catcgccagg accatcaatg aggtagagaa ccagatcctg acccgggatg ccaagggcat
     2281 cagccaggag cagatgaatg agttccgggc ctccttcaac cactttgacc gggatcactc
     2341 cggcacactg ggtcccgagg agttcaaagc ctgcctcatc agcttgggtt atgatattgg
     2401 caacgacccc cagggagaag cagaatttgc ccgcatcatg agcattgtgg accccaaccg
     2461 cctgggggta gtgacattcc aggccttcat tgacttcatg tcccgcgaga cagccgacac
     2521 agatacagca gaccaagtca tggcttcctt caagatcctg gctggggaca agaactacat
     2581 taccatggac gagctgcgcc gcgagctgcc acccgaccag gctgagtact gcatcgcgcg
     2641 gatggccccc tacaccggcc ccgactccgt gccaggtgct ctggactaca tgtccttctc
     2701 cacggcgctg tacggcgaga gtgacctcaa gcttcgaatt ctgcagtcga cggtaccgcg
     2761 gccccgggat ccaccggtcg ccaccatggt gagcaagggc gaggggcaat cgaagcatgg
     2821 actaaaggag gagatgacgg tgaagtacca catggaaggt tgcgttaatg gtcacaaatt
     2881 tgtcattact ggagagggca ttggaaaccc ttttaagggt aagcaaaccg caaatttgtg
     2941 tgtgatagaa ggaggcccgc tgccgttctc ggaggacatt ttaagtccgg gttttaaata
     3001 tggtgaccgg attttcacag agtacccaca ggatattgta gattacttca agaactcatg
     3061 tccagcgggc tatacgtggg aaaggagcta cctctttgag gacggagcgg tctgtcgatg
     3121 caacgtggac ataacagtct ctgaaaagga gaactgcatc tatcacaaaa gtatcttcag
     3181 aggggtgaat tttcccgccg acggccccgt aatgaagaaa atgaccacta attgggaagc
     3241 tagtaccgag aaaattgtgc ctgttccaaa gcaagggata ttaaagggaa aggtcaaaat
     3301 gtgcctgttg ctgaaggatg gcggtcgtta tcattgccag tttgatacgg tatataaagc
     3361 taaatcagtg ccctccaaga tgccagaatg gcattttata cagcataagc tccttaggga
     3421 ggaccgctct gatgctaaaa accagaaatg gcaattaaca gaacatgcaa tcgcaggcat
     3481 ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat accacattt
//
