LOCUS       sequence-147116-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human SSRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_SSRP1
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..109)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(125..148)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(152..175)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(179..202)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccaggtccag
       61 ggttctcctc cacgtctcca gcctgcttca gcaggctgaa gttagtagct ccgcttcctg
      121 aaaccttatc gtcgtcatcc ttgtaatctc ccttatcgtc gtcatccttg taatcgccct
      181 tatcgtcgtc atccttgtaa tctccagaac cacctccacc ggaacctcct ccgctcccct
      241 catcggatcc tgacgctgag tcctctgagc tggggggagt actggctagt tcttcttctt
      301 cagagtcctg aaaaccaaag gtgtcttaag ggaaaagctg gagtcctggg tgccccaccc
      361 actcttcagt gaagggcccg caattcccta ccgcccccaa ctacagcacc ccccttcaga
      421 acctcagaat tccaccatcc actccaactc attactctta cagaccaacc ccacacagac
      481 acacatgccc ctcaccctgc ccagcccaca ggccccacat tcctgccgca cctcgctcct
      541 cctcctcttc tttttgctct tgttctctcc cgaagagctc tcatcactag acacaaactc
      601 tttgctcttg aagctctcgc ttagctgcct tgaggacgac ttggatgatg agcccctaga
      661 gggcgtggat ttcttttcca tctttacctt tactttcttc ttcttctttg acttgtccct
      721 gtattggcaa gagggaagag gagctgggcc ttcaggtccc ctgtcaccat gaccaaacct
      781 ctcccagctt taacaaggcc tttcccaatc ccagccacac agcccatttc actctgaacg
      841 taaatagctg cgtcagcctg gagggcacca agggagagta tcatgagtac accagaagaa
      901 tcagagggca cagcctaaag cagcccaatg tgcaaaacct acttgatatt tcttgcttta
      961 tcttttagag ttcatgcaaa ctgtgcactg cactccataa tagtattttt ttcgttcccc
     1021 cataccttca aataaa
//
