LOCUS       sequence-147118-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mNanog Homeodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-mNanogHD-pgk-hph
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..47
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(62..67,68..85,86..92)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     124..140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(408..432)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(483..502)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(564..586)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
       61 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      121 acacaggaaa cagctatgac catgattacg ccaagctatc accactttgt acaagaaagc
      181 tgggtcttac aaccactggt ttttctgcca ccgcttgcac ttcatccttt ggttttgaaa
      241 ccaggtctta acctgcttat agctcaggtt cagaatggag gagagttctt gcatctgctg
      301 gaggctgagg tacttctgct tctgaaacct gtccttgagt gcacacagct gggcctgaga
      361 gaacacagtc cgcatcttct gcttcctggc aaggaccttc atggtgaagc ctgctttttt
      421 gtacaaactt gtgatagctt atcgataccg tcgaggaatt ctttgccaaa atgatgagac
      481 agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg
      541 ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc cctgccccgt
      601 cccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg gagaagggga
      661 cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac
      721 cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt cggctccgca
      781 cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa aaggcaatgg
      841 ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg gccgccgcgc ccctcccccg
      901 agccctcccc ggcc
//
