LOCUS       sequence-147120-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mNanog Homeodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-mNanogHD-pgk-hph
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(321..345)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(366..382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(379..401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    390..406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(414..420,421..438,439..444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    459..480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(540..557)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    546..770
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    847..880
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgacggta
       61 tcgataagct atcacaagtt tgtacaaaaa agcaggcttc accatgaagg tccttgccag
      121 gaagcagaag atgcggactg tgttctctca ggcccagctg tgtgcactca aggacaggtt
      181 tcagaagcag aagtacctca gcctccagca gatgcaagaa ctctcctcca ttctgaacct
      241 gagctataag caggttaaga cctggtttca aaaccaaagg atgaagtgca agcggtggca
      301 gaaaaaccag tggttgtaag acccagcttt cttgtacaaa gtggtgatag cttggcgtaa
      361 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      421 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      481 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatcagc
      541 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      601 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
      661 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
      721 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
      781 ggaaagaacc agctggggct cgagatccac tagttctagc ctcgaggcta gagcggccgc
      841 gtcgagataa cttcgtataa tgtatgctat acgaagttat gttttgttac tttatagaag
      901 aaattttgag tttttgtttt tttttaataa ataaat
//
