LOCUS       sequence-147121-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton , N1 Cloning Vector, Excitation:505 / 
            569, Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-MYR-N-5
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..82)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(155..358)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(184..204)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        359..662
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tacggagttt gatcttcatg tctggcttaa tcgcactcat ggtggcgacc ggtccctttg
       61 gcttgctctt gctgcttccc atggtggcga attcgaagct tgagctcgag atctgagtcc
      121 ggtagcgcta gcggatctga cggttcacta aaccagctct gcttatatag acctcccacc
      181 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      241 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
      301 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
      361 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
      421 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
      481 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
      541 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
      601 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
      661 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
      721 taatgcatgg cggtaatacg gttatccac
//
