LOCUS       sequence-147130-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral empty cloning vector containing IRES and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG3 (pCMV-IRES-GFP version 3)
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(36..44,45..47,48..621)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(209..228)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     438..455
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(772..796)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(793..844)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(822..842)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga gatctggttg tggcaagctt atcatcgtgt
       61 ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga
      121 ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatggggtac
      181 cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc
      241 cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta
      301 tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca
      361 aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac
      421 gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt
      481 caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga
      541 aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt
      601 aggggggggg agggagaggg gcggatcccc ttaattaacc ttcgaaccgc gatcgcccgg
      661 cgcgccccgt ttaaacccga tatcccctcg agccgtatac cctgatcacc gaattcgcta
      721 gctctagaat cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg
      781 gttcactaaa cgagctctgc ttatataaac ctcccaccgt acacgcctac cgcccatttg
      841 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
      901 caaactccca ttgacgtcaa tggggtggag acttggaaat cccc
//
