LOCUS       sequence-147133-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  image recycling of VGAT in live neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Santos MS, Park CK, Foss SM, Li H, Voglmaier SM
  TITLE     Sorting of the vesicular GABA transporter to functional vesicle 
            pools by an atypical dileucine-like motif.
  JOURNAL   J Neurosci. 2013 Jun 26;33(26):10634-46. doi: 
            10.1523/JNEUROSCI.0329-13.2013.
  PUBMED    23804087
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0329-13"; journalName: "J Neurosci"; date: 
            "2013-06-26- 26"; volume: "33"; issue: "26"; pages: "10634-46"
COMMENT     Sequence Label: vGAT-pH pcaggs SE
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             277..990
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(304..332)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     927..947
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgcgcgctgg tggtcttcac gctgctcatg gccatctacg tgccacactt cgcgctgctc
       61 atgggcctca cgggcagcct cacgggagcc ggcctctgct tcctgctgcc cagcctcttc
      121 cacttgcgtc ttctctggcg caagctgctg tggcaccagg tcttcttcga tgtggccatc
      181 ttcgtcatcg gcggcatctg cagcgtgtcc ggcttcgtgc attcactcga gggcctcatc
      241 gaggcctacc gaaccaacgc agaggacacc ggtggaagta aaggagaaga acttttcact
      301 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      361 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      421 actggaaaac tacctgttcc ttggccaaca cttgtcacta ctttaactta tggtgttcaa
      481 tgcttttcaa gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc
      541 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      601 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      661 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa cgatcaccag
      721 gtgtacatca tggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattagacac
      781 aacattgaag atggaggcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      841 gatgggcccg tccttttacc agacaaccat tacctgttta caacttctac tctttcgaaa
      901 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      961 acacatggca tggatgaact atacaaataa
//
