LOCUS       sequence-147137-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mKlf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   dos Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, 
            Hendrich B, Silva JC
  TITLE     MBD3/NuRD facilitates induction of pluripotency in a 
            context-dependent manner.
  JOURNAL   Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 
            10.1016/j.stem.2014.04.019. Epub 2014 May 15.
  PUBMED    24835571
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2014.04.019"; journalName: "Cell Stem Cell"; date: 
            "2014-07-3- 3"; volume: "15"; issue: "1"; pages: "102-10"
COMMENT     Sequence Label: pPB-CAG-Klf4-pA-pgk-hph
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..51)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    59..75
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(83..89,90..107,108..113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    128..149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(209..226)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    215..439
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    516..549
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(772..791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(773..791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(801..818)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(801..817)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(810..832)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      959..1414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1046..1065)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1256..1277
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1440..1544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1545..1613,1614..2405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1763..1782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2576..3164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3065..3084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3318..3335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3452..3473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3488..3493,3494..3511,3512..3518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3526..3542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3550..3566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3585..3605
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3587..3605
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   3628..3662
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(4145..5170)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(4145..5170)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5189..5688)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     5630..5653
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    5711..5744
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        5760..6139
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6142..6417
                     /label=chicken beta-actin promoter
     intron          6418..7426
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7350..7372
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7434..7453
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    7504..7528
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(9008..9018,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
       61 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      181 cgggaaacct gtcgtgccag cggatcagcc tcgactgtgc cttctagttg ccagccatct
      241 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      301 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      361 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      421 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gagatccact
      481 agttctagcc tcgaggctag agcggccgcg tcgagataac ttcgtataat gtatgctata
      541 cgaagttatg ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
      601 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
      661 taaaacttaa tatctattca aattaataaa taaacgtcaa ttttacgcat gattatcttt
      721 aacgtacgtc acaatatgat tatctttcta gggttaaaga gctccaattc gccctatagt
      781 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      841 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      901 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
      961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     1201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     1321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     1381 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     1441 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1501 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1561 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1621 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1681 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1741 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1801 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1861 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1921 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1981 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2041 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2101 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2161 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2221 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2281 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2341 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2401 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2701 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3241 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3361 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3421 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3481 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3541 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     3601 aaagggaaca aaagctgggt accttaaccc tagaaagata gtctgcgtaa aattgacgca
     3661 tgcattcttg aaatattgct ctctctttct aaatagcgcg aatccgtcgc tgtgcattta
     3721 ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc
     3781 actgattttg aactataacg accgcgtgag tcaaaatgac gcatgattat cttttacgtg
     3841 acttttaaga tttaactcat acgataatta tattgttatt tcatgttcta cttacgtgat
     3901 aacttattat atatatattt tcttgttata gatatccaac tatgaaacat tatcataaag
     3961 caatagtaaa gagccttcag taaagagcag gcatttatct aatcccaccc cacccccacc
     4021 cccgtagctc caatccttcc attcaaaatg taggtactct gttctcaccc ttcttaacaa
     4081 agtatgacag gaaaaaactt ccatgtggac atctttattg tttaatagat catcaatttc
     4141 tcgactattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     4201 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     4261 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     4321 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     4381 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     4441 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     4501 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     4561 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     4621 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     4681 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     4741 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     4801 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     4861 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     4921 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     4981 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     5041 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     5101 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     5161 gctttttcat ggtggcggct ggatcggtcg aaaggcccgg agatgaggaa gaggagaaca
     5221 gcgcggcaga cgtgcgcttt tgaagcgtgc gagaatgccg ggcctccgga ggaccttcgg
     5281 gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc
     5341 ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc
     5401 cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc
     5461 atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga
     5521 ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg
     5581 gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc agcggggctg
     5641 ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaattcat
     5701 cgatgatatc ataacttcgt ataatgtatg ctatacgaag ttatgcggcc ctcgaggtcg
     5761 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     5821 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5881 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5941 tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg cagtacatca
     6001 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     6061 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     6121 agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     6181 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     6241 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
     6301 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     6361 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     6421 gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg cccgccccgg
     6481 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     6541 tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg tgaaagcctt
     6601 aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg cgtgcgtgtg
     6661 tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga gcgctgcggg
     6721 cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc gggggcggtg
     6781 ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
     6841 ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg cacccccctc
     6901 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg cgtggcgcgg
     6961 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     7021 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg gcggctgtcg
     7081 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     7141 tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     7201 gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     7261 cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc agggggacgg
     7321 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     7381 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     7441 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgacggta tcgataagct
     7501 atcacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat ggctgtcagc
     7561 gacgctctgc tcccgtcctt ctccacgttc gcgtccggcc cggcgggaag ggagaagaca
     7621 ctgcgtccag caggtgcccc gactaaccgt tggcgtgagg aactctctca catgaagcga
     7681 cttcccccac ttcccggccg cccctacgac ctggcggcga cggtggccac agacctggag
     7741 agtggcggag ctggtgcagc ttgcagcagt aacaacccgg ccctcctagc ccggagggag
     7801 accgaggagt tcaacgacct cctggaccta gactttatcc tttccaactc gctaacccac
     7861 caggaatcgg tggccgccac cgtgaccacc tcggcgtcag cttcatcctc gtcttcccca
     7921 gcgagcagcg gccctgccag cgcgccctcc acctgcagct tcagctatcc gatccgggcc
     7981 gggggtgacc cgggcgtggc tgccagcaac acaggtggag ggctcctcta cagccgagaa
     8041 tctgcgccac ctcccacggc ccccttcaac ctggcggaca tcaatgacgt gagcccctcg
     8101 ggcggcttcg tggctgagct cctgcggccg gagttggacc cagtatacat tccgccacag
     8161 cagcctcagc cgccaggtgg cgggctgatg ggcaagtttg tgctgaaggc gtctctgacc
     8221 acccctggca gcgagtacag cagcccttcg gtcatcagtg ttagcaaagg aagcccagac
     8281 ggcagccacc ccgtggtagt ggcgccctac agcggtggcc cgccgcgcat gtgccccaag
     8341 attaagcaag aggcggtccc gtcctgcacg gtcagccggt ccctagaggc ccatttgagc
     8401 gctggacccc agctcagcaa cggccaccgg cccaacacac acgacttccc cctggggcgg
     8461 cagctcccca ccaggactac ccctacactg agtcccgagg aactgctgaa cagcagggac
     8521 tgtcaccctg gcctgcctct tcccccagga ttccatcccc atccggggcc caactaccct
     8581 cctttcctgc cagaccagat gcagtcacaa gtcccctctc tccattatca agagctcatg
     8641 ccaccgggtt cctgcctgcc agaggagccc aagccaaaga ggggaagaag gtcgtggccc
     8701 cggaaaagaa cagccaccca cacttgtgac tatgcaggct gtggcaaaac ctataccaag
     8761 agttctcatc tcaaggcaca cctgcgaact cacacaggcg agaaacctta ccactgtgac
     8821 tgggacggct gtgggtggaa attcgcccgc tccgatgaac tgaccaggca ctaccgcaaa
     8881 cacacagggc accggccctt tcagtgccag aagtgcgaca gggccttttc caggtcggac
     8941 caccttgcct tacacatgaa gaggcacttt taaatcccac gtagtggatg taagggtggg
     9001 cgcgccgacc cagctttc
//
