LOCUS       sequence-147139-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of zNanog Homeodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-zNanogHD-pgk-hph
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    52..76
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(335..359)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(380..396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(380..396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(393..415)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    404..420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(428..434,435..452,453..458))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    473..494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(554..571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    560..784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gctgtctcat cattttggca aagaattcct cgacggtatc gataagctat cacaagtttg
       61 tacaaaaaag caggcttcac catgcggcct ccgaccctgc cccgcaagac ccgggctgct
      121 ttttctgaag aacagatgaa tgctttggtc aatcggttta atgtccagag atacctcacg
      181 cctgcagaga tgaaaacgct tgctggagca acaggattaa catacaagca ggtaaaaaca
      241 tggtttcaga accgcaggat gaaactcaag aggcatcaga gagacagcag ttggatgact
      301 gagagatatg ttgtcaatgc tgtacccaac taagacccag ctttcttgta caaagtggtg
      361 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      421 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      481 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      541 gccagcggat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      661 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
      721 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
      781 atggcttctg aggcggaaag aaccagctgg ggctcgagat ccactagttc tagcctcgag
      841 gctagagcgg ccgcgtcgag ataacttc
//
