LOCUS       sequence-147140-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of zNanog Homeodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-zNanogHD-pgk-hph
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..47
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(62..67,68..85,86..92)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     124..140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(444..468)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(519..538)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(600..622)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
       61 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      121 acacaggaaa cagctatgac catgattacg ccaagctatc accactttgt acaagaaagc
      181 tgggtcttag ttgggtacag cattgacaac atatctctca gtcatccaac tgctgtctct
      241 ctgatgcctc ttgagtttca tcctgcggtt ctgaaaccat gtttttacct gcttgtatgt
      301 taatcctgtt gctccagcaa gcgttttcat ctctgcaggc gtgaggtatc tctggacatt
      361 aaaccgattg accaaagcat tcatctgttc ttcagaaaaa gcagcccggg tcttgcgggg
      421 cagggtcgga ggccgcatgg tgaagcctgc ttttttgtac aaacttgtga tagcttatcg
      481 ataccgtcga ggaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc
      541 aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg
      601 gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc
      661 cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga cgcacgaagg
      721 ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg cccgctagag
      781 ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa aggaagtccc
      841 tgcgccctct cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag
      901 ccgccggcgc tccggggccg ccgcgcccct ccc
//
