LOCUS       sequence-147146-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-TMEM216-N-18
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(393..596)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(422..442)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tacggagttt gatcctcatg tctggcttaa tcgcactcac catggtggcg accggtggat
       61 cccgggcccg cggtaccgtc gactgcagaa ttcgagagat cctgtccatg ctggagaagg
      121 tggccagcgt gagcatctca agcagcagct ctgagccaca aaagaagagc aagatactgt
      181 tcatgatagc ttctaggcgg agcacgtagg tctgcagcag caggtaatag gaagccatca
      241 tagcggatgg gaaggtcaag gccacactaa tgccaagggg catctttcgc tggcagaggt
      301 ttccctttgt accgaaaaac aatcgtatga cttcaatgcc aagatagagc agcagcatgg
      361 tggcgctagc ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt
      421 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
      481 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
      541 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
      601 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
      661 tactgggcat aatgccacgc gggccattta ccgtcattga cgtcaata
//
