LOCUS       sequence-147152-         528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C with C-terminal 
            BirA-biotinylation signal and TEV celavage site .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2/C - AVITEV
FEATURES             Location/Qualifiers
     source          1..528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(78..94)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(79..95)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(128..172)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(185..205)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg gggggcggaa
       61 ttcgatatca agcttatcga taccgtcgac ctcgacgcgg ccgcttataa gcgatcgctc
      121 agttaacctc atgccattcg attttctgtg cctcgaagat atcgttcagg ccaccgccgt
      181 taacgccctg gaagtacagg ttctctacgt acgtctgatt tctggcagga ggtcctgaaa
      241 actccttcct gataatttca ttaacatcat ccaagtaggt ctggtccaag ttctgctcct
      301 gtttaatggt cacccccgca ggtaatactt ccttttggtc actgaccggg ggtccgtttt
      361 tggcggctct ttggctcgtg gcattctgct gctgaatgac tgtggggtag gaacccgggc
      421 tcagcctctg accttgactg accgggggcg ggccaggtct ggtggtgcct ggtgcgaaat
      481 tctcgcagta catgatgtgc tggaactcct ggtggcttcc gcagcgca
//
