LOCUS       sequence-147154-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag tagged mNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-Flag3-pG-mNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..35
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             44..109
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ataagctatc acaagtttgt acaaaaaagc aggcttcacc atggactaca aagaccatga
       61 cggtgattat aaagatcatg atatcgatta caaggatgac gatgacaagg gatccggcgg
      121 cggcggcggc gccatgagtg tgggtcttcc tggtccccac agtttgccta gttctgagga
      181 agcatcgaat tctgggaacg cctcatcaat gcctgcagtt tttcatcccg agaactattc
      241 ttgcttacaa gggtctgcta ctgagatgct ctgcacagag gctgcctctc ctcgcccttc
      301 ctctgaagac ctgcctcttc aaggcagccc tgattcttct accagtccca aacaaaagct
      361 ctcaagtcct gaggctgaca agggccctga ggaggaggag aacaaggtcc ttgccaggaa
      421 gcagaagatg cggactgtgt tctctcaggc ccagctgtgt gcactcaagg acaggtttca
      481 gaagcagaag tacctcagcc tccagcagat gcaagaactc tcctccattc tgaacctgag
      541 ctataagcag gttaagacct ggtttcaaaa ccaaaggatg aagtgcaagc ggtggcagaa
      601 aaaccagtgg ttgaagacta gcaatggtct gattcagaag ggctcagcac cagtggagta
      661 tcccagcatc cattgcagct atccccaggg ctatctggtg aacgcatctg gaagcctttc
      721 catgtggggc agccagactt ggaccaaccc aacttggagc agccagacct ggaccaaccc
      781 aacttggaac aaccagacct ggaccaaccc aacttggagc agccaggcct ggaccgctca
      841 gtcctggaac ggccagcctt ggaatgctgc tccgctccat aacttcgggg aggactttct
      901 gcagccttac gtacagttgc agcaaaactt
//
