LOCUS       sequence-147155-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag tagged mNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-Flag3-pG-mNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..46
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(61..66,67..84,85..91)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    99..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     104..126
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     123..139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     123..139
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
       61 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      121 cacaggaaac agctatgacc atgattacgc caagctatca ccactttgta caagaaagct
      181 gggtctcata tttcacctgg tggagtcaca gagtagttca ggaataattc caaggcttgt
      241 ggggtgctaa aatgcgcatg gctttcccta gtggcttcca aattcacctc caaatcactg
      301 gcagagaagt tttgctgcaa ctgtacgtaa ggctgcagaa agtcctcccc gaagttatgg
      361 agcggagcag cattccaagg ctggccgttc caggactgag cggtccaggc ctggctgctc
      421 caagttgggt tggtccaggt ctggttgttc caagttgggt tggtccaggt ctggctgctc
      481 caagttgggt tggtccaagt ctggctgccc cacatggaaa ggcttccaga tgcgttcacc
      541 agatagccct ggggatagct gcaatggatg ctgggatact ccactggtgc tgagcccttc
      601 tgaatcagac cattgctagt cttcaaccac tggtttttct gccaccgctt gcacttcatc
      661 ctttggtttt gaaaccaggt cttaacctgc ttatagctca ggttcagaat ggaggagagt
      721 tcttgcatct gctggaggct gaggtacttc tgcttctgaa acctgtcctt gagtgcacac
      781 agctgggcct gagagaacac agtccgcatc ttctgcttcc tggcaaggac cttgttctcc
      841 tcctcctcag ggcccttgtc agcctcagga cttgagagct tttgtttggg actggtagaa
      901 gaatcagggc tgccttgaag aggcaggtct tcagaggaag ggcgaggaga ggcagcctct
      961 gtgcagagca tctcagtagc agacccttgt aagcaagaat agttctcggg atgaaaaact
     1021 gcaggcattg atgaggcgtt cccagaattc gatgcttcct cagaactagg caaactgtgg
     1081 ggaccaggaa gaccc
//
