LOCUS       sequence-147156-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, N1 Cloning Vector, Excitation:487, 
            Emission:509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CENPA-N-22
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    461..513
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      521..530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(572..593)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagccacc atgggcccgc gccgccggag
       61 cctaaagccc gaggccccga ggaggcgcag cccgagcccg accccgaccc ccggcccctc
      121 ccggcggggc ccctccttag gcgcttcctc ccatcaacac agtcggcgga gacaaggttg
      181 gctaaaggag atccgaaagc ttcagaagag cacacacctc ttgataagga agctgccctt
      241 cagccgcctg gcaagagaaa tatgtgttaa attcactcgt ggtgtggact tcaattggca
      301 agcccaggcc ctattggccc tacaagaggc agcagaagca tttctagttc atctctttga
      361 ggacgcctat ctcctcacct tacatgcagg ccgagttact ctcttcccaa aggatgtgca
      421 actggcccgg aggatccggg gccttgagga gggactcggc ctcgagctca agcttcgaat
      481 tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg
      541 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      601 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      661 gaagttcatc tgcac
//
