LOCUS       sequence-147158-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression vector for expression of StrepOne-Tagged human
            BCL10 (AS 1-116).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMSCV40-EF1a-hBCL10(1-114)-SO
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             414..437
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             474..497
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     513..529
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     577..593
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aatcactgct tactggctta tcgaaattaa tacgactcac tatagggtct agacccacaa
       61 tggagcccac cgcaccgtcc ctcaccgagg aggacctcac tgaagtgaag aaggacgcct
      121 tagaaaattt acgtgtatac ctgtgtgaga aaatcatagc tgagagacat tttgatcatc
      181 tacgtgcaaa aaaaatactc agtagagaag acactgaaga aatttcttgt cgaacatcaa
      241 gtagaaaaag ggctggaaaa ttgttagact acttacagga aaacccaaaa ggtctggaca
      301 cccttgttga atctattcgg cgagaaaaaa cacagaactt cctgatacag aagattacag
      361 atgaagtgct gaaacttaga aatataaaac tagaacatct gaaaggaagc gcttggagcc
      421 acccgcagtt cgagaaaggt ggaggttccg gaggtggatc gggaggtgga tcgtggagcc
      481 acccgcagtt cgaaaaataa taagcttgcg gtcgaggtcg acggtatcga taagcttgat
      541 atcgaattcc ggcggccgcg gaccggttaa ttaactcgag gtcgacggta tcgataagct
      601 tgatatcgaa ttccgccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
      661 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
      721 cccggaa
//
