LOCUS       sequence-147159-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression vector for expression of StrepOne-Tagged human
            BCL10 (AS 1-116).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMSCV40-EF1a-hBCL10(1-114)-SO
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(69..85)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(133..149)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(165..188)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(225..248)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(615..634)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(616..634)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggccttatt ccaagcggct tcggccagta acgttagggg ggggggcgga attcgatatc
       61 aagcttatcg ataccgtcga cctcgagtta attaaccggt ccgcggccgc cggaattcga
      121 tatcaagctt atcgataccg tcgacctcga ccgcaagctt attatttttc gaactgcggg
      181 tggctccacg atccacctcc cgatccacct ccggaacctc cacctttctc gaactgcggg
      241 tggctccaag cgcttccttt cagatgttct agttttatat ttctaagttt cagcacttca
      301 tctgtaatct tctgtatcag gaagttctgt gttttttctc gccgaataga ttcaacaagg
      361 gtgtccagac cttttgggtt ttcctgtaag tagtctaaca attttccagc cctttttcta
      421 cttgatgttc gacaagaaat ttcttcagtg tcttctctac tgagtatttt ttttgcacgt
      481 agatgatcaa aatgtctctc agctatgatt ttctcacaca ggtatacacg taaattttct
      541 aaggcgtcct tcttcacttc agtgaggtcc tcctcggtga gggacggtgc ggtgggctcc
      601 attgtgggtc tagaccctat agtgagtcgt attaatttcg ataagccagt aagcagtgat
      661 ttaaatgtcg aaattcctca cgacacctga aatggaagaa aa
//
