LOCUS       sequence-147160-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag tagged cNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-Flag3-pG-cNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..39
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             48..113
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atcgataagc tatcacaagt ttgtacaaaa aagcaggctt caccatggac tacaaagacc
       61 atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac aagggatccg
      121 gcggcggcgg cggcgccatg ccgtcctacg gctctgttag gtgcggacgc tactactggc
      181 cctctccggg cagcatggat agcgcgtctg ccgcggaagc cccagcagca gacctctcct
      241 tgaccacaga gcagaaaacg ccctgccacc cagatgcctc tccagcttct tccagctctg
      301 ggacactcat tcagtatacc ccagactctg ccactagccc tactgcagac cacccatctc
      361 accgccccac ttttcagaag gttaaggata aaggtgagag tgggacaagg aaggccaaga
      421 gccgcacagc tttctcccag gagcagctgc agaccctgca ccagcggttt cagagccaga
      481 agtacctcag cccccatcag atccgggagc tggctgctgc tctggggctc acctacaagc
      541 aggtgaagac gtggtttcag aaccaacgaa tgaagtttaa acgttgccag aaagagagtc
      601 agtgggtgga taaagggatt tatctaccac agaatgggtt tcatcaagct gcgtatctgg
      661 atatgacccc tacgtttcac cagggcttcc ctgttgttgc caacagaaac cttcaggctg
      721 tgaccagtgc acaccaggct tacagcagtg gccagactta tgggaatggg cagggcctgt
      781 atccgttcat ggctgtggag gatgagggct tctttggaaa aggtggaaca agctgcaacg
      841 cccagcaggc catgggttta ttaagtcaac agatgaactt ctatcatggc tactctacca
      901 atgtggatta tgacagcttg
//
