LOCUS       sequence-147161-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag tagged cNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-Flag3-pG-cNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..45
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(60..65,66..83,84..90)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    98..114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     103..125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     122..138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     122..138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
       61 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      121 acaggaaaca gctatgacca tgattacgcc aagctatcac cactttgtac aagaaagctg
      181 ggtcctaagt ctcataacca ttctgggtcc ccagggtatg ccaaggggcc tggtactgat
      241 gccgtacagg agagctcgag aactgtgtga tactatcaga ggtgctctgg aagctgtagg
      301 tatcttctgc ctgcaagctg tcataatcca cattggtaga gtagccatga tagaagttca
      361 tctgttgact taataaaccc atggcctgct gggcgttgca gcttgttcca ccttttccaa
      421 agaagccctc atcctccaca gccatgaacg gatacaggcc ctgcccattc ccataagtct
      481 ggccactgct gtaagcctgg tgtgcactgg tcacagcctg aaggtttctg ttggcaacaa
      541 cagggaagcc ctggtgaaac gtaggggtca tatccagata cgcagcttga tgaaacccat
      601 tctgtggtag ataaatccct ttatccaccc actgactctc tttctggcaa cgtttaaact
      661 tcattcgttg gttctgaaac cacgtcttca cctgcttgta ggtgagcccc agagcagcag
      721 ccagctcccg gatctgatgg gggctgaggt acttctggct ctgaaaccgc tggtgcaggg
      781 tctgcagctg ctcctgggag aaagctgtgc ggctcttggc cttccttgtc ccactctcac
      841 ctttatcctt aaccttctga aaagtggggc ggtgagatgg gtggtctgca gtagggctag
      901 tggcagagtc tggggtatac tgaatgagtg tcccagagct ggaagaagct ggagaggcat
      961 ctgggtggca gggcgttttc tgctctgtgg tcaaggagag gtctgctgct ggggcttccg
     1021 cggcagacgc gctatccatg ctgcccggag agggccagta gtagcgtccg cacctaaca
//
