LOCUS       sequence-147163-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 
            / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Alpha-V-Integrin-25
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..449)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     regulatory      446..455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    465..517
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgctt atccatccag ctgcactccg atgaaatact ggagctcacc cttctggtcc
      181 ctcatgggct ggaggtgcag gaggttccag aatttcttcc cgctttttgt gtagtttatc
      241 agctgcacgg taatctcgcg ctgatctcta atggcatcgc gaatcttctg cactgtggcc
      301 tggtctgtct ctggtccctg cagaaagcgg ccattgcggc ccaggatctc ctctctggaa
      361 tactcggtca gctccaggaa gccatcggat gcgaagatga tgggattgtc tggcagccgt
      421 ggatcagtta tcacgaaact tttctccatg gtggcgaccg gtggggatcc cgggcccgcg
      481 gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc ccggagtttc tgagtttcct
      541 tcaccatttt catgaggttg aagctgctcc ctttcttgtt cttcttgagg tggccggacc
      601 cgtttaaaaa agcccatcct gtacattaca aataccaaaa cagccagtag caacaatcct
      661 gctagaactg ctaaaatgat cacccacaca ggcacaggca tgggcgctgg ctgaatgccc
      721 caggtgacat tagtggtaac caatgtggag ttggtgatat cctcaattgg aagattctta
      781 taaggaaact ctatgacatt aaatgaagca gacgacttca gagaatagga atgattctga
      841 ttttctttat tcataa
//
