LOCUS       sequence-147166-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of aBsx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-aBsx-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..40
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(55..60,61..78,79..85)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    93..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     98..120
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     117..133
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     117..133
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    154..178
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(800..824)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(875..894)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(956..978)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
       61 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      121 aaacagctat gaccatgatt acgccaagct atcaccactt tgtacaagaa agctgggtct
      181 cagtcctctg tgtctgaggc gacctcagga ctcgcacaga tgtctagttt gtcatggatc
      241 gtcgcctggt gctcgggtct ggcaacatcg tctgccgaag aggccgacgg cggtctgtcg
      301 ctttctctct tcctctggag cttcttgtgc ttcatacgtc tgttttggaa ccacgtcttc
      361 acctgtgttt ctgtgaggtt gagtgcggcg gcgaggtcca ttctctccgg tgtggacagg
      421 tatttctgac tctcgaaccg cttctccaga ccgttcagct gcgagtccga gaacaccgtc
      481 cgggcctttc gccggcggat cggctttccg gggaacccgc cgttatggta caacggggac
      541 atcgggatcc catggctgtg tagaagatag tgagagtgca gacccggctt gtggaggtag
      601 gggtggaagc cgtgcgggag gaaggcggag ggtgccggtg ccagcacgcc ggggaaaccc
      661 acctccacca gagagttccg tgtgggctgc ggcaccacca actcgccgtg ccgaggacag
      721 tccttgtgct tgtcaaacag aatgttctcg atcaggaaag ctgtcgtgtt gccgctctgc
      781 gactgggaga acatggtgaa gcctgctttt ttgtacaaac ttgtgatagc ttatcgatac
      841 cgtcgaggaa ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga
      901 gctgtaggaa aaagaagaag gcatgaacat ggttagcaga ggctctagag ccgccggtca
      961 cacgccagaa gccgaacccc gccctgcccc gtcccccccg aaggcagccg tccccc
//
