LOCUS       sequence-147168-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GESTALT V1 integrating barcode with Puro and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLJM1-EGFP-BarcodeV1
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    335..452
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        501..984
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     930..948
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tgagactggg ggtggaggtt tagcagtgcg gctagaggtg gtggaatggc actggggctg
       61 ggggaggcgg ttaggcactg tggctgcagg tggtggataa gcactgcagc tgggagtgga
      121 ggtatggcac tgagaccaga ggtggtggaa atagcactgt ggctggggga ggtggtatac
      181 ggctgtgcgg ccagaggtgg aggaataggc cacagcggcc ggaggtggca gttctaggca
      241 atgtggctga aggtggagga tctagccact gcagctagag gtggaggata gtcgaattct
      301 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      361 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      421 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      481 cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
      541 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
      601 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
      661 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
      721 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
      781 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
      841 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
      901 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
      961 acgtcggcag tcggctccct cgttgaccga atcaccga
//
