LOCUS       sequence-147169-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for selfcleaving E.coli biotin ligase 
            BirA-P2A-mCherry fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMSCV-BirA-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgcggccgc gccaccatgg agaaggataa caccgtgcca ctgaaattga ttgccctgtt
       61 agcgaacggt gaatttcact ctggcgagca gttgggtgaa acgctgggaa tgagccgggc
      121 ggctattaat aaacacattc agacactgcg tgactggggc gttgatgtct ttaccgttcc
      181 gggtaaagga tacagcctgc ctgagcctat ccagttactt aatgctaaac agatattggg
      241 tcagctggat ggcggtagtg tagccgtgct gccagtgatt gactccacga atcagtacct
      301 tcttgatcgt atcggagagc ttaaatcggg cgatgcttgc attgcagaat accagcaggc
      361 tggccgtggt cgccggggtc ggaaatggtt ttcgcctttt ggcgcaaact tatatttgtc
      421 gatgttctgg cgtctggaac aaggcccggc ggcggcgatt ggtttaagtc tggttatcgg
      481 tatcgtgatg gcggaagtat tacgcaagct gggtgcagat aaagttcgtg ttaaatggcc
      541 taatgacctc tatctgcagg atcgcaagct ggcaggcatt ctggtggagc tgactggcaa
      601 aactggcgat gcggcgcaaa tagtcattgg agccgggatc aacatggcaa tgcgccgtgt
      661 tgaagagagt gtcgttaatc aggggtggat cacgctgcag gaagcgggga tcaatctcga
      721 tcgtaatacg ttggcggcca tgctaatacg tga
//
