LOCUS       sequence-147172-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for selfcleaving E.coli biotin ligase 
            BirA-P2A-mCherry fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMSCV-BirA-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..151)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
       61 tggccatgtt atcctcctcg cccttgctca ccatagggcc ggggttctcc tccacgtcgc
      121 cggcctgctt cagcaggctg aagttggtgg cgccgctgcc tttttctgca ctacgcaggg
      181 atatttcacc gcccatccag ggttttatta ttccatcctg ctcaagtaat aaagccccct
      241 gtttgtctat tccgcgtgaa atgccaaata tttctttatc accaatgata agtttcactg
      301 ggcgattaat aaaattatcc agcttttccc agcgcgacag ataaggtgcc aatccttctt
      361 gttcgaagag ttccaacgca gcacgtaatt cacgtattag catggccgcc aacgtattac
      421 gatcgagatt gatccccgct tcctgcagcg tgatccaccc ctgattaacg acactctctt
      481 caacacggcg cattgccatg ttgatcccgg ctccaatgac tatttgcgcc gcatcgccag
      541 ttttgccagt cagctccacc agaatgcctg ccagcttgcg atcctgcaga tagaggtcat
      601 taggccattt aacacgaact ttatctgcac ccagcttgcg taatacttcc gccatcacga
      661 taccgataac cagacttaaa ccaatcgccg ccgccgggcc ttgttccaga cgccagaaca
      721 tcgacaaata taagtttgcg ccaa
//
