LOCUS       sequence-147178-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GESTALT V2 integrating barcode with Puro and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLJM1-EGFP-BarcodeV2
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    264..381
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        430..940
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     859..877
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(962..981)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgtgcggcca gaggtggagg aataggccac tgcgactgga ggaggtggat actggcactg
       61 cagcctgggg gtgaggtaga tgccacagcg gccggaggtg gcagttctag gcacggaggc
      121 tggaagtggt ggtgaaaggc actggggcag gagatggagg actttgtcac tgcggctgca
      181 gatggtggga attggctctt cggctggagg tagaggctaa tcgaattctc gacctcgaga
      241 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      301 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      361 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
      421 cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
      481 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
      541 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
      601 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
      661 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
      721 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
      781 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
      841 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
      901 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac cggagcttac
      961 catgaccgag tacaagccca cgg
//
