LOCUS       sequence-147180-        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mNanog (Y42E, K43T).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-mNanog-mut-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..47
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(62..67,68..85,86..92)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     124..140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
       61 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      121 acacaggaaa cagctatgac catgattacg ccaagctatc accactttgt acaagaaagc
      181 tgggtctcat atttcacctg gtggagtcac agagtagttc aggaataatt ccaaggcttg
      241 tggggtgcta aaatgcgcat ggctttccct agtggcttcc aaattcacct ccaaatcact
      301 ggcagagaag ttttgctgca actgtacgta aggctgcaga aagtcctccc cgaagttatg
      361 gagcggagca gcattccaag gctggccgtt ccaggactga gcggtccagg cctggctgct
      421 ccaagttggg ttggtccagg tctggttgtt ccaagttggg ttggtccagg tctggctgct
      481 ccaagttggg ttggtccaag tctggctgcc ccacatggaa aggcttccag atgcgttcac
      541 cagatagccc tggggatagc tgcaatggat gctgggatac tccactggtg ctgagccctt
      601 ctgaatcaga ccattgctag tcttcaacca ctggtttttc tgccaccgct tgcacttcat
      661 cctttggttt tgaaaccagg tcttaacctg ggtctcgctc aggttcagaa tggaggagag
      721 ttcttgcatc tgctggaggc tgaggtactt ctgcttctga aacctgtcct tgagtgcaca
      781 cagctgggcc tgagagaaca cagtccgcat cttctgcttc ctggcaagga ccttgttctc
      841 ctcctcctca gggcccttgt cagcctcagg acttgagagc ttttgtttgg gactggtaga
      901 agaatcaggg ctgccttgaa gaggcaggtc ttcagaggaa gggcgaggag aggcagcctc
      961 tgtgcagagc atctcagtag cagacccttg taagcaagaa tagttctcgg gatgaaaaac
     1021 tgcaggcatt gatgaggcgt tcccagaatt cgatgcttcc tcagaactag gcaaactgtg
     1081 gggaccagga agacccacac tcatggtgaa gcctgctttt t
//
