LOCUS       sequence-147181-         496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgLacZ-1, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pXPR_BRD003 sgLacZ-1
FEATURES             Location/Qualifiers
     source          1..496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        80..155
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    211..328
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
       61 acggcggatt gaccgtaatg ttttagagct agaaatagca agttaaaata aggctagtcc
      121 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc gtaactagat
      181 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      241 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      301 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
      361 ttggcgccgg ctcgaggggg cccgggtgca aagatggata aagttttaaa cagagaggaa
      421 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
      481 tcagtgggca gagcgc
//
