LOCUS       sequence-147184-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GESTALT V3 integrating barcode with Puro and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLJM1-EGFP-BarcodeV3
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    271..388
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        437..947
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     866..884
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(970..989)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aatcggcact ggggctgggg gtgcaggaat agtgcactgc agctgcaggt ggaggatact
       61 ggaacggcgg ccggaggtgt aggtagatga cactgtggct ggaagtggaa gttctaggca
      121 caggggctga aggtgatggt gaaatgctct gcggcaggag gaggaggact ttgacactaa
      181 ggctggaggt ggggagaatt ggcactgcgg ccagagggag tggctaatcg aattctcgac
      241 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      301 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      361 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
      421 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
      481 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
      541 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
      601 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
      661 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
      721 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
      781 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
      841 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
      901 cggcagtcgg ctccctcgtt gancgaatca ccgacctctc tccccagggg gatccaccng
      961 gagcttacca tgaccgagta caagcccacg gtgc
//
