LOCUS       sequence-147185-        8365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GESTALT V4 integrating barcode with Puro and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLJM1-EGFP-BarcodeV4
FEATURES             Location/Qualifiers
     source          1..8365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      373..382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(379..381,382..384,385..1095)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(379..381,382..384,385..1095)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(424..445)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(685..704)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1032..1053
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1448..1565
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1614..2124
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2043..2061
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2146..2745
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2146..2165)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2642..2662
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2873..3106
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3178..3299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3215..3234)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3269..3288
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3339..3474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3494..3513)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3495..3513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3523..3540)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3523..3539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3532..3554)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3681..4136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3768..3787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3978..3999
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4162..4266
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4267..4335,4336..5127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4485..4504)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5298..5886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5787..5806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6040..6057
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6174..6195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6210..6215,6216..6233,6234..6240)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6248..6264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6253..6275
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6272..6288
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6272..6288
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6307..6327
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6309..6327
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6355..6581
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6582..6762
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6809..6934
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7427..7660
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7845..7889
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8187..8207
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      421 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      481 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      541 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      601 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      661 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      721 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      781 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      841 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      901 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      961 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1021 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1081 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgggcaa tgtggctgaa
     1141 ggtggaggaa atcggcacag cagctggagg tgctggaata ggccactgca gctagaggtg
     1201 gaggatacta gcactggggc tgggggaggt ggtagatggc tctgtggccg gaggaggagg
     1261 ttctaggcac ggaggctgga agtggtggtg aaagtcactg cggctgcaga tggaggactt
     1321 tgacacaacg gcaggaggtg gtgggaatta gcacagcggc tggagaagga agctaatggc
     1381 cctgcggccg ggggtggtga ttgaacgaat tctcgacctc gagacaaatg gcagtattca
     1441 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1501 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1561 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
     1621 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     1681 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     1741 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     1801 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     1861 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     1921 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     1981 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     2041 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     2101 cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag
     2161 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     2221 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     2281 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     2341 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     2401 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     2461 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     2521 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     2581 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     2641 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     2701 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     2761 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg
     2821 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     2881 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     2941 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3001 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3061 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     3121 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3181 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3241 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3301 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3361 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3421 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     3481 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     3541 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3601 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3661 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3721 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3781 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3841 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3901 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3961 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4021 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4081 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4141 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4201 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4261 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4321 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4381 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4441 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4501 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4561 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4621 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4681 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4741 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4801 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4861 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4921 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4981 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5041 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5101 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5161 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5221 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5281 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5341 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5401 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5461 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5521 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5581 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5641 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5701 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5761 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5821 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5881 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5941 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6001 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6061 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6121 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6181 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6241 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6301 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6361 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     6421 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     6481 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     6541 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     6601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     6781 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     6841 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     6901 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     6961 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7021 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7081 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7141 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7201 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7261 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7321 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7381 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     7441 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     7501 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     7561 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     7621 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     7681 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     7741 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     7801 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     7861 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     7921 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     7981 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     8041 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     8101 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     8161 agcctcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc
     8221 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     8281 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     8341 aattatgttt taaaatggac tatca
//
