LOCUS       sequence-147186-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GESTALT V4 integrating barcode with Puro and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLJM1-EGFP-BarcodeV4
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    294..411
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        460..970
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     889..907
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 gcacngcagc tggaggtgct ggaataggcc actgcagcta gaggtggagg atactagcac
       61 tggggctggg ggaggtggta gatggctctg tggccggagg aggaggttct aggcacggag
      121 gctggaagtg gtggtgaaag tcactgcggc tgcagatgga ggactttgac acaacggcag
      181 gaggtggtgg gaattagcac agcggctgga gaaggaagct aatggccctg cggccggggg
      241 tggtgattga acgaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
      301 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      361 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
      421 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
      481 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
      541 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
      601 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
      661 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
      721 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
      781 ggccaatagc ggctgctcag canggcgcgc cgagagcagc ggccgggaag gggcggtgcg
      841 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
      901 ctgcaagcct ccggancgca cgtcngcagt cggctccctc gttgaccgaa tcancgacct
      961 ctctccccag ggggatcc
//
