LOCUS       sequence-147190-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgLacZ-2, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pXPR_BRD003 sgLacZ-2
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        36..111
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        442..653
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 aaggacgaaa caccgctaac gcctgggtcg aacgcgtttt agagctagaa atagcaagtt
       61 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttaag
      121 cttggcgtaa ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccactttgg cgccggctcg agggggcccg ggtgcaaaga tggataaagt
      361 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      421 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      481 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
      541 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      601 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      661 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      721 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtg
//
