LOCUS       sequence-147204-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgATXN1L-2, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pXPR_BRD003 sgATXN1L-2
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..122
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        202..277
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    333..450
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
       61 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      121 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      181 gagacccaat gaactgcagc ggttttagag ctagaaatag caagttaaaa taaggctagt
      241 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg gcgtaactag
      301 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
      361 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      421 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      481 ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg
      541 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
      601 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      661 aattgatc
//
