LOCUS       sequence-147208-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mouse cofilin fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Chai X, Zhao S, Fan L, Zhang W, Lu X, Shao H, Wang S, Song L, Failla
            AV, Zobiak B, Mannherz HG, Frotscher M
  TITLE     Reelin and cofilin cooperate during the migration of cortical 
            neurons: a quantitative morphological analysis.
  JOURNAL   Development. 2016 Mar 15;143(6):1029-40. doi: 10.1242/dev.134163. 
            Epub 2016 Feb 18.
  PUBMED    26893343
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.134163"; journalName: "Development"; date: "2016-03-15-
            15"; volume: "143"; issue: "6"; pages: "1029-40"
COMMENT     Sequence Label: pCAG-cofilin WT
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      554..563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(596..615)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(677..699)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcagatctc tcgagcagcg ctgctcgagg caagcttcgc
       61 aaaggcttgc cctccaggga aatgacggcg ctgccaccta gtttctctgc cagggtgcag
      121 cggtccttga cctcctcgta gcagttagct tgtaattcat gcttgattcc tgtcagcttc
      181 ttcttgatgg catccttgga gctggcatag atcattttgc tcttgagggg tgcattctcg
      241 ggggcccaga agatgaacac caggtcctcc ttcttgctct ccttggtctc ataggttgca
      301 tcatagagtg catagcggca gtccttgtct ggcagcatct tgacaaaagt ggtgtagggg
      361 tcgtccacag tctgccccac atctcctacc aggatctcct tgccctcctc caggatgatg
      421 ttcttcttgt cctcactcag gcaaaagagc accgccttct tgcgtttctt cacttcttct
      481 ggtgttgaag acttgcgaac cttcatgtca ttgaacacct tgatgacacc atcagagaca
      541 gccacaccag aggccatggt ggcgaattca attctttgcc aaaatgatga gacagcacaa
      601 taaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca tggttagcag
      661 aggctctaga gccgccggtc acacgccaga agccgaaccc cgccctgccc cgtccccccc
      721 gaaggcagcc gtccccccgc ggacagcccc gaggctggag agggagaagg ggacggcggc
      781 gcggcgacgc acgaaggccc tccccgccca tttccttcct gccggcgccg caccgctt
//
