LOCUS       sequence-147210-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mouse cofilin S3A (nonphosphorylatable form)
            fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Chai X, Zhao S, Fan L, Zhang W, Lu X, Shao H, Wang S, Song L, Failla
            AV, Zobiak B, Mannherz HG, Frotscher M
  TITLE     Reelin and cofilin cooperate during the migration of cortical 
            neurons: a quantitative morphological analysis.
  JOURNAL   Development. 2016 Mar 15;143(6):1029-40. doi: 10.1242/dev.134163. 
            Epub 2016 Feb 18.
  PUBMED    26893343
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.134163"; journalName: "Development"; date: "2016-03-15-
            15"; volume: "143"; issue: "6"; pages: "1029-40"
COMMENT     Sequence Label: pCAG-cofilin-S3A
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      551..560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(602..623)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agattgattc gccaccatgg ccgctggtgt ggctgtctct gatggtgtca tcaaggtgtt
       61 caatgacatg aaggttcgca agtcttcaac accagaagaa gtgaagaaac gcaagaaggc
      121 ggtgctcttt tgcctgagtg aggacaagaa gaacatcatc ctggaggagg gcaaggagat
      181 cctggtagga gatgtggggc agactgtgga cgacccctac accacttttg tcaagatgct
      241 gccagacaag gactgccgct atgcactcta tgatgcaacc tatgagacca aggagagcaa
      301 gaaggaggac ctggtgttca tcttctgggc ccccgagaat gcacccctca agagcaaaat
      361 gatctatgcc agctccaagg atgccatcaa gaagaagctg acaggaatca agcatgaatt
      421 acaagctaac tgctacgagg aggtcaagga ccgctgcacc ctggcagaga aactaggtgg
      481 cagcgccgtc atttccctgg agggcaagcc tttgcgaagc ttgcctcgag cagcgctgct
      541 cgagagatct gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      601 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      661 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      721 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      781 ccccgaccac atgaagcagc acgact
//
