LOCUS       sequence-147211-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mouse cofilin S3A (nonphosphorylatable form)
            fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Chai X, Zhao S, Fan L, Zhang W, Lu X, Shao H, Wang S, Song L, Failla
            AV, Zobiak B, Mannherz HG, Frotscher M
  TITLE     Reelin and cofilin cooperate during the migration of cortical 
            neurons: a quantitative morphological analysis.
  JOURNAL   Development. 2016 Mar 15;143(6):1029-40. doi: 10.1242/dev.134163. 
            Epub 2016 Feb 18.
  PUBMED    26893343
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.134163"; journalName: "Development"; date: "2016-03-15-
            15"; volume: "143"; issue: "6"; pages: "1029-40"
COMMENT     Sequence Label: pCAG-cofilin-S3A
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(589..608)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(670..692)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ttgctaccat ggtggcagat ctctcgagca gcgctgctcg aggcaagctt cgcaaaggct
       61 tgccctccag ggaaatgacg gcgctgccac ctagtttctc tgccagggtg cagcggtcct
      121 tgacctcctc gtagcagtta gcttgtaatt catgcttgat tcctgtcagc ttcttcttga
      181 tggcatcctt ggagctggca tagatcattt tgctcttgag gggtgcattc tcgggggccc
      241 agaagatgaa caccaggtcc tccttcttgc tctccttggt ctcataggtt gcatcataga
      301 gtgcatagcg gcagtccttg tctggcagca tcttgacaaa agtggtgtag gggtcgtcca
      361 cagtctgccc cacatctcct accaggatct ccttgccctc ctccaggatg atgttcttct
      421 tgtcctcact caggcaaaag agcaccgcct tcttgcgttt cttcacttct tctggtgttg
      481 aagacttgcg aaccttcatg tcattgaaca ccttgatgac accatcagag acagccacac
      541 cagcggccat ggtggcgaat tcaattcttt gccaaaatga tgagacagca caataaccag
      601 cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct
      661 agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca
      721 gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga
      781 cgcacgaagg ccctcc
//
