LOCUS       sequence-147215-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mouse Gnptg with C-terminal His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Encarnacao M, Kollmann K, Trusch M, Braulke T, Pohl S
  TITLE     Post-translational modifications of the gamma-subunit affect 
            intracellular trafficking and complex assembly of 
            GlcNAc-1-phosphotransferase.
  JOURNAL   J Biol Chem. 2011 Feb 18;286(7):5311-8. doi: 
            10.1074/jbc.M110.202382. Epub 2010 Dec 20.
  PUBMED    21173149
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.202382"; journalName: "J Biol Chem"; date: 
            "2011-02-18- 18"; volume: "286"; issue: "7"; pages: "5311-8"
COMMENT     Sequence Label: pcDNA3.1-mGnptg
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
       61 ggctagcgtt taaacttaag cttgacccta ggagcaatgg cggggcggct ggctggcttc
      121 ctgatgttgc tggggctcgc gtcgcagggg cccgcgccgg catgtgccgg gaagatgaag
      181 gtggtggagg agcctaacac attcgggctg aataacccgt tcttgcccca ggcaagccgc
      241 cttcagccca agagagagcc ttcagctgta tccgggcccc tgcatctctt cagacttgct
      301 ggcaagtgct ttagcctagt ggagtccacg tacaagtatg aattctgccc tttccacaac
      361 gtcacccagc acgagcagac cttccgctgg aacgcctaca gcgggatcct tggcatctgg
      421 catgagtggg aaatcatcaa caataccttc aagggcatgt ggatgactga tggggactcc
      481 tgccactccc ggagccggca gagcaaggtg gagctcacct gtggaaagat caaccgactg
      541 gcccacgtgt ctgagccaag cacctgtgtc tatgcattga cattcgagac ccctcttgtt
      601 tgccatcccc actctttgtt agtgtatcca actctgtcag aggccctgca gcagcgctgg
      661 gaccaggtgg aacaggacct ggcagacgaa ctgatcacac cacagggcta tgagaagttg
      721 ctaagggtac ttt
//
