LOCUS       sequence-147220-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of LbNOX which 
            is a water-forming NADH oxidase that can be used to increase 
            NAD+/NADH ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Titov DV, Cracan V, Goodman RP, Peng J, Grabarek Z, Mootha VK
  TITLE     Complementation of mitochondrial electron transport chain by 
            manipulation of the NAD+/NADH ratio.
  JOURNAL   Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. 
            Epub 2016 Apr 7.
  PUBMED    27124460
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad4017"; journalName: "Science"; date: "2016-04-8-
            8"; volume: "352"; issue: "6282"; pages: "231-5"
COMMENT     Sequence Label: pUC57-LbNOX
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..35)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agacgcgttt acttgtcatc gtcatccttg taatccatag atccaccaga tccaccagat
       61 ccaccagcgt tgacggattg ggccacttta gcttgggcag cctgggcgag aatgttcagg
      121 tagttgaagg gcctgtcgaa attgggctgg aacagcatgt ccaccatggc caggtcatcg
      181 atggtgttct cgttctggat gcagacggac agggtattag cgctctggga cacgtcgtat
      241 ttggacatca gggcgcctcc caggattctg tgtgtatcgg ggtcgaagac caggctcatg
      301 aggacgggct cggtggaagg catgaactcg ggcctatagt tgtcctccac gatgacctgc
      361 tcggcattga ggccttgttg cttggcggcg gccagtgtga ggcctgtgga gacaattgtc
      421 ctgtcataga gggccagtcc ggagctggac tgggttccca tgtacttcac tgtgggcttg
      481 acgaggttct ttccgacgag gattccctgc ctgacggcgt tggtagcgag gggaatgtag
      541 gcgttttggt gtgtggggtt gtaatggacg gcggcggagt cgccagcagc gaaaatatca
      601 gggttgctgg acctcatgta gtcgtcggta atgatggcgc cgttaggggc catgtccact
      661 ttgcctttca gcaggtctgt gttgggccta aagccaatgc agaggatggc caggtcggtc
//
