LOCUS       sequence-147225-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of rNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-rNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..45
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(60..65,66..83,84..90)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    98..114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     103..125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     122..138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     122..138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
       61 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      121 acaggaaaca gctatgacca tgattacgcc aagctatcac cactttgtac aagaaagctg
      181 ggtctcatat ttcgcctgga gaattcacgg agtagttcag gaacaattcc aaggcttgcg
      241 gggtactaaa atgcgcctgg ctttccctag tggcttccaa attcgcctcc aaatcactgg
      301 cggagaagtt ttgctgcaac ggcacataag gctgcaggga gtcctccccg aagttatgga
      361 gcggagcagc gttccaagtc tggctgttcc aggcctgagt acaccaggac tgagtgctcc
      421 aggcctggtt gctccaagtt gggttggtcc aggtctggtt gctccaggtt gggttggtcc
      481 aggtctggtt gttccaagtt gggttggtcc aggtctgact gccccatact ggaaggtttc
      541 cagacgcgtt catcagatag ccctgagaat agctgcaatg gatgctggga tactccaccg
      601 gcgctgagcc cttctgagtc aggccgttgc tagtcttcaa ccattggttt ttctgccacc
      661 tcttgcactt cattctttgg ttttggaacc aggtcttcac ctgcttatag ctcaggttca
      721 gaatggtaga gagatcttgc atctgctgga ggctgaggta cctttgcctc tgaaacctat
      781 ccttgagtgc acacaactgg gcctgagaga acacagtccg catcttctgc ttcttggtga
      841 ggaccttgtt ctcttctttc ttctcagggc cctcgtcagc ctcgggacca gacagcttta
      901 gcttgggatt gctagaagaa tcagggctat cttgaagagg taggtcccca gaggaaggcg
      961 gaggagaggc agtctctgtg cagagcatct cagtagcaga cacttgtaag caagaataat
     1021 tttcctcagg aagatgaacg gcagg
//
