LOCUS       sequence-147230-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mNanog .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Theunissen TW, van Oosten AL, Castelo-Branco G, Hall J, Smith A, 
            Silva JC
  TITLE     Nanog overcomes reprogramming barriers and induces pluripotency in 
            minimal conditions.
  JOURNAL   Curr Biol. 2011 Jan 11;21(1):65-71. doi: 10.1016/j.cub.2010.11.074. 
            Epub 2010 Dec 30.
  PUBMED    21194951
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2010.11.074"; journalName: "Curr Biol"; date: 
            "2011-01-11- 11"; volume: "21"; issue: "1"; pages: "65-71"
COMMENT     Sequence Label: pPB-CAG-Nanog-pA-pgk-hph
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    75..99
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgcacggt
       61 atcgataagc tatcacaagt ttgtacaaaa aagcaggctt caccatgagt gtgggtcttc
      121 ctggtcccca cagtttgcct agttctgagg aagcatcgaa ttctgggaac gcctcatcaa
      181 tgcctgcagt ttttcatccc gagaactatt cttgcttaca agggtctgct actgagatgc
      241 tctgcacaga ggctgcctct cctcgccctt cctctgaaga cctgcctctt caaggcagcc
      301 ctgattcttc taccagtccc aaacaaaagc tctcaagtcc tgaggctgac aagggccctg
      361 aggaggagga gaacaaggtc cttgccagga agcagaagat gcggactgtg ttctctcagg
      421 cccagctgtg tgcactcaag gacaggtttc agaagcagaa gtacctcagc ctccagcaga
      481 tgcaagaact ctcctccatt ctgaacctga gctataagca ggttaagacc tggtttcaaa
      541 accaaaggat gaagtgcaag cggtggcaga aaaaccagtg gttgaagact agcaatggtc
      601 tgattcagaa gggctcagca ccagtggagt atcccagcat ccattgcagc tatccccagg
      661 gctatctggt gaacgcatct ggaagccttt ccatgtgggg cagccagact tggaccaacc
      721 caacttggag cagccagacc tggaccaacc caacttggaa caaccagacc tggaccaacc
      781 caacttggag cagccaggcc tggaccgctc agtcctggaa cggccagcct tggaatgctg
      841 ctccgctcca taacttcggg gaggactttc tgcagcctta cgtacagttg cagcaaaact
      901 tctctgccag tgatttggag gtgaatttgg
//
