LOCUS       sequence-147235-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mNanog .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Theunissen TW, van Oosten AL, Castelo-Branco G, Hall J, Smith A, 
            Silva JC
  TITLE     Nanog overcomes reprogramming barriers and induces pluripotency in 
            minimal conditions.
  JOURNAL   Curr Biol. 2011 Jan 11;21(1):65-71. doi: 10.1016/j.cub.2010.11.074. 
            Epub 2010 Dec 30.
  PUBMED    21194951
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2010.11.074"; journalName: "Curr Biol"; date: 
            "2011-01-11- 11"; volume: "21"; issue: "1"; pages: "65-71"
COMMENT     Sequence Label: pPB-CAG-Nanog-pA-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..45
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(60..65,66..83,84..90)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    98..114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     103..125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     122..138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     122..138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
       61 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      121 acaggaaaca gctatgacca tgattacgcc aagctatcac cactttgtac aagaaagctg
      181 ggtctcatat ttcacctggt ggagtcacag agtagttcag gaataattcc aaggcttgtg
      241 gggtgctaaa atgcgcatgg ctttccctag tggcttccaa attcacctcc aaatcactgg
      301 cagagaagtt ttgctgcaac tgtacgtaag gctgcagaaa gtcctccccg aagttatgga
      361 gcggagcagc attccaaggc tggccgttcc aggactgagc ggtccaggcc tggctgctcc
      421 aagttgggtt ggtccaggtc tggttgttcc aagttgggtt ggtccaggtc tggctgctcc
      481 aagttgggtt ggtccaagtc tggctgcccc acatggaaag gcttccagat gcgttcacca
      541 gatagccctg gggatagctg caatggatgc tgggatactc cactggtgct gagcccttct
      601 gaatcagacc attgctagtc ttcaaccact ggtttttctg ccaccgcttg cacttcatcc
      661 tttggttttg aaaccaggtc ttaacctgct tatagctcag gttcagaatg gaggagagtt
      721 cttgcatctg ctggaggctg aggtacttct gcttctgaaa cctgtccttg agtgcacaca
      781 gctgggcctg agagaacaca gtccgcatct tctgcttcct ggcaaggacc ttgttctcct
      841 cctcctcagg gcccttgtca gcctcaggac ttgagagctt ttgtttggga ctggtagaag
      901 aatcagggct gccttgaaga ggcaggtctt cagaggaagg gcgaggagag gcagcctctg
      961 tgcagagcat ctcagtagca gacccttgta agcaagaata gttctcggga tgaaaaactg
     1021 caggcattga tgaggcgttc ccagaattcg atgctt
//
