LOCUS       sequence-147253-        2179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_J23100. [F:36INS-J23100:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 41 to 75)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-J23100_FB
FEATURES             Location/Qualifiers
     source          1..2179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        41..75
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    87..107
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(113..130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(284..872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(364..383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1043..1834,1835..1903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1666..1685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1904..2008)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2076..2094
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2098..2141)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2144..2165
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 cgctcctgca gtattcattt tcagcttacg gaaggtagat ttgacggcta gctcagtcct
       61 aggtacagtg ctagctacta gagacctact agtagcggcc gctgcaggct tcctcgctca
      121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
      301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
      961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1021 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1081 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1141 gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca cgctcaccgg
     1201 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     1261 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     1321 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     1381 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     1441 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     1501 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     1561 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     1621 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     1681 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     1741 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     1801 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     1861 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     1921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     1981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     2041 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt
     2101 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc
     2161 tagagactag tgggtctca
//
