LOCUS       sequence-147262-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal HA-tagged NEK7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-HA-NEK7
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             664..672
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgcatgctc gagttaggtg cttgcggtac atgcatgcat cctctttgcc acatcataaa
       61 cataggcgat gtcgggtcgc ttctctggat ctgggttgat gcatatatta actagctgtc
      121 gtagctcctc cgaatagtga tctgacggga gaggcgggta gtcacactgc tctatcttct
      181 tacacagaga atacaagttc atcttgtcgc cgtagaaagg actctgcagt gcagccatct
      241 catatagcag acagccaaga gaccagatgt cagacttgaa gttgtatcca ttttcatgta
      301 ttctctctgg agacatgtag taaggtgtac ccactaaaga atgtgcggct gtggttttgg
      361 agctgaaaaa ccgaccaagc ccaaggtctc cgagttttac taccccagtg gctgtaatga
      421 acacattagc tggttttata tctctgtgca tgactcttcg agaatgcatg tggtccagtg
      481 cactgcagag ctgaacgaag tatttccaaa cggttctctc agggattagc ctcttttgtt
      541 tcttaaagtg ctttatcatt ctggagaggt caccagcatc tgctaactcc aaaactatgt
      601 tcagctcatt atcctcaatg aatgatgcat agtatttaat tacatttggg tggttgagtt
      661 gcttaaggag gtctatttct ttgatacaat cagcacgtgc ttt
//
