LOCUS       sequence-147265-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; 
            kinase dead) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-HA-NEK7 K64M
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             667..675
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tagatgcatg ctcgagttag gtgcttgcgg tacatgcatg catcctcttt gccacatcat
       61 aaacataggc gatgtcgggt cgcttctctg gatctgggtt gatgcatata ttaactagct
      121 gtcgtagctc ctccgaatag tgatctgacg ggagaggcgg gtagtcacac tgctctatct
      181 tcttacacag agaatacaag ttcatcttgt cgccgtagag aggactctgc agtgcagcca
      241 tctcatatag cagacagcca agagaccaga tgtcagactt gaagttgtat ccattttcat
      301 gtattctctc tggagacatg tagtaaggtg tacccactaa agaatgtgca gctgtggttt
      361 tggagctgaa aaaccgacca agcccaaggt ctccgagttt tactacccca gtggctgtaa
      421 tgaacacatt agctggtttt atatctctgt gcatgactct tcgagaatgc atgtggtcca
      481 gtgcactgca gagctgaacg aagtatttcc aaacggttct ctcagggatt agcctctttt
      541 gtttcttaaa gtgctttatc attctggaga ggtcaccagc atctgctaac tccaaaacta
      601 tgttcagctc attatcctca atgaatgatg catagtattt aattacattt ggatggttga
      661 gttgcttaag gaggtctatt tctttgatac aatcagcacg tgcttt
//
