LOCUS       sequence-147266-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged pro-IL-1-beta in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pCMV-pro-Il1b-C-Flag
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(181..246)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 acttgcccct tgctccatac cacccccctc caccccataa tattatagaa ggacacctag
       61 tcagacaaaa tgatgcaact taattttatt aggacaaggc tggtgggcac tggagtggca
      121 acttccaggg ccaggagagg cactggggag gggtcacagg gatgccaccc gggatcacta
      181 cttgtcatcg tcatccttgt agtcgatgtc atgatcttta taatcaccgt catggtcttt
      241 gtagtcagcc cgggatcctc tagaggaaga cacggattcc atggtgaagt caattatgtc
      301 ctgaccactg ttgtttccca ggaagacagg cttgtgctct gcttgtgagg tgctgatgta
      361 ccagttgggg aactctgcag actcaaactc cactttgctc ttgacttcta tcttgttgaa
      421 gacaaaccgt ttttccatct tcttctttgg gtattgcttg ggatccacac tctccagctg
      481 cagggtgggt gtgccgtctt tcattacaca ggacaggtat agattctttc ctttgaggcc
      541 caaggccaca ggtattttgt cgttgcttgg ttctccttgt acaaagctca tggagaatat
      601 cacttgttgg ttgatattct gtccattgag gtggagagct ttcagctcat atgggtccga
      661 cagcacgagg cttttttgtt gtttatctcg gagcctgtag tgcagttgtc taatgggaac
      721 gtcacacacc agcaggttat catcatcatc ccatgagtca cagaggatgg gctcttcttc
      781 aaagatgaag gaaaagaagg tgctcatgtc ctcatcctgg aaggtccacg ggaaaaaga
//
