LOCUS       sequence-147268-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged ASC1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-ASC1
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(629..655)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(677..696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(678..696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagtcagct ctgctccagg tccatcacca
       61 agtagggatg tatttccttc aaggcctgga ggagggagtc cttgcaggtc aggttccagg
      121 atggaacaaa gctgaagagc ttcctcatct tgtcttggct ggtggtctct gcacgaactg
      181 cctggtactg tccttcagtc agcacactgc catgcaaagc atccagcact ccgtccactt
      241 ctgtgaccct ggcaatgagt gcttgcctgt gctggtccac aaagtgtcct gttctggctg
      301 tactctgagc agggacactg gctgcagctg ccacagctcc agactcttct ttagtcgttt
      361 gcagctgctc agccagctcc tgtaagccca tgtctctaag cacagtcatt gtgagctcca
      421 agccatacga ctccagatag tagctgacaa gtttgtcagt gagatctatg gcgtccatct
      481 gcagcagggc cccgcgtggg atgcgcccat agccttctcg cagttgcact gtcagcagct
      541 tcatcttgga ctttttgagt tcatcccctg acaagttttc aagagcgtcc aggatggcat
      601 ctcgtgcccg ccccatgaat tcggatccgg cgtagtcggg gacgtcgtag gggtatccca
      661 tggtaagctt gggtctccct atagtgagtc gtattaattt cgataa
//
