LOCUS       sequence-147278-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mammalian cell codon-optimized dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK
  TITLE     CRISPR RNA-guided activation of endogenous human genes.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.
  PUBMED    23892898
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598."
COMMENT     Sequence Label: pMLM3705
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac
       61 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg
      121 gacataaacc gtttatctga ttacgacgtc gatgccattg taccccaatc ctttttgaag
      181 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac
      241 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat
      301 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg
      361 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca
      421 aagcatgttg cacagatact agattcccga atgaatacga aatacgacga gaacgataag
      481 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag
      541 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat
      601 cttaatgccg tcgtagggac cgcactcatt aa
//
