LOCUS       sequence-147279-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mammalian cell codon-optimized dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK
  TITLE     CRISPR RNA-guided activation of endogenous human genes.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.
  PUBMED    23892898
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598."
COMMENT     Sequence Label: pMLM3705
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg ccaccatgga
       61 taaaaagtat tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac
      121 cgatgaatac aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc
      181 gattaaaaag aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac
      241 tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt
      301 acaagaaatt tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga
      361 agagtccttc cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat
      421 agtagatgag gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct
      481 agttgactca actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat
      541 aaagttccgt gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga
      601 caaactgttc atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa
      661 tgcaagtggc gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct
      721 agaaaacctg atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat
      781 agcgctctca ctaggcctga cacc
//
